One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays (Record no. 77756)
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| 000 -LEADER | |
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| fixed length control field | 04600nam a22006015i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-981-13-8094-5 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220801215703.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190513s2019 si | s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| ISBN | 9789811380945 |
| -- | 978-981-13-8094-5 |
| 082 04 - CLASSIFICATION NUMBER | |
| Call Number | 610.28 |
| 100 1# - AUTHOR NAME | |
| Author | Song, Seo Woo. |
| 245 10 - TITLE STATEMENT | |
| Title | One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2019. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | XXI, 52 p. 46 illus., 40 illus. in color. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Springer Theses, Recognizing Outstanding Ph.D. Research, |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Remark 2 | Abstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the ‘Pipetting-Free’ HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients’ primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of ‘partipetting’ for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://doi.org/10.1007/978-981-13-8094-5 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | eBooks |
| 264 #1 - | |
| -- | Singapore : |
| -- | Springer Nature Singapore : |
| -- | Imprint: Springer, |
| -- | 2019. |
| 336 ## - | |
| -- | text |
| -- | txt |
| -- | rdacontent |
| 337 ## - | |
| -- | computer |
| -- | c |
| -- | rdamedia |
| 338 ## - | |
| -- | online resource |
| -- | cr |
| -- | rdacarrier |
| 347 ## - | |
| -- | text file |
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| -- | rda |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Biomedical engineering. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Bioinformatics. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Soft condensed matter. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Microtechnology. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Microelectromechanical systems. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Industrial microbiology. |
| 650 14 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Biomedical Engineering and Bioengineering. |
| 650 24 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Computational and Systems Biology. |
| 650 24 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Soft and Granular Matter. |
| 650 24 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Microsystems and MEMS. |
| 650 24 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Industrial Microbiology. |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| -- | 2190-5061 |
| 912 ## - | |
| -- | ZDB-2-ENG |
| 912 ## - | |
| -- | ZDB-2-SXE |
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