Includes bibliographical references and index.

Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.

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Intro; Title Page; Copyright; Table of Contents; Foreword; Preface; Part I: Hydrodynamic Flow Confinement (HFC); Chapter 1: Hydrodynamic Flow Confinement Using a Microfluidic Probe; 1.1 Introduction; 1.2 HFC Principle; 1.3 MFP Heads; 1.4 Vertical MFP; 1.5 Advanced MFP Heads and Holders; 1.6 Surface Processing Using an MFP; 1.7 MFP Components; 1.8 Outlook; Acknowledgments; References; Chapter 2: Hierarchical Hydrodynamic Flow Confinement (hHFC) and Recirculation for Performing Microscale Chemistry on Surfaces; 2.1 Introduction; 2.2 Hierarchical HFC; 2.3 Recirculation; 2.4 Microscale Deposition.

AcknowledgmentsReferences; Chapter 3: Design of Hydrodynamically Confined Microflow Devices with Numerical Modeling: Controlling Flow Envelope, Pressure, and Shear Stress; 3.1 Introduction; 3.2 Theory; 3.3 Device and Experimental Methods for CFD Validation; 3.4 Numerical Modeling of HCM devices; 3.5 Envelope Size and Pressure Drop Across HCMs; 3.6 Hydrodynamic Loads Generated by HCM Devices; 3.7 Concluding Remarks; References; Chapter 4: Hele-Shaw Flow Theory in the Context of Open Microfluidics: From Dipoles to Quadrupoles; 4.1 Introduction; 4.2 Fundamentals of Hele-Shaw Flows.

4.3 Applications to Microfluidic Dipoles and Quadrupoles4.4 Diffusion in Hele-Shaw Flows; 4.5 Conclusion; References; Chapter 5: Implementation and Applications of Microfluidic Quadrupoles; 5.1 Introduction; 5.2 Principles and Configurations of MQs; 5.3 Implementation of MQs; 5.4 MQ Analysis and Characterization; 5.5 Application of MQs in Biology and Life Sciences; 5.6 Summary and Outlook; References; Chapter 6: Hydrodynamic Flow Confinement-Assisted Immunohistochemistry from Micrometer to Millimeter Scale; 6.1 Immunohistochemical Analysis of Tissue Sections.

6.2 Probe Heads for Multiscale Surface Interactions6.3 Immunohistochemistry with Microfluidic Probes; 6.4 Micro-IHC on Human Tissue Sections; 6.5 Millimeter-Scale Immunohistochemistry; 6.6 Outlook; Acknowledgments; References; Chapter 7: Local Nucleic Acid Analysis of Adherent Cells; 7.1 Introduction; 7.2 Methods; 7.3 Results; 7.4 Discussion; 7.5 Concluding Remarks; Acknowledgments; References; Chapter 8: Microfluidic Probe for Neural Organotypic Brain Tissue and Cell Perfusion; 8.1 Introduction; 8.2 Microperfusion of Organotypic Brain Slices Using the Microfluidic Probe.

8.3 Microperfusion of Live Dissociated Neural Cell Cultures Using the Microfluidic Probe8.4 Conclusion; Acknowledgments; References; Chapter 9: The Multifunctional Pipette; 9.1 Introduction; 9.2 Open Volume Probes; 9.3 Detailed View on the Multifunctional Pipette; 9.4 Integrated Functions; 9.5 Functional Extensions and Applications; 9.6 Future Technology; Acknowledgments; References; Chapter 10: Single-Cell Analysis with the BioPen; 10.1 Introduction; 10.2 The Single-Cell Challenge; 10.3 Superfusion Techniques; 10.4 The BioPen; 10.5 Application Areas; 10.6 Future Technology; Acknowledgments.