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Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis [electronic resource] / by Bin Zhuang.

By: Zhuang, Bin [author.].
Contributor(s): SpringerLink (Online service).
Material type: materialTypeLabelBookSeries: Springer Theses, Recognizing Outstanding Ph.D. Research: Publisher: Singapore : Springer Nature Singapore : Imprint: Springer, 2018Edition: 1st ed. 2018.Description: XXIII, 114 p. 58 illus., 44 illus. in color. online resource.Content type: text Media type: computer Carrier type: online resourceISBN: 9789811047534.Subject(s): Biomedical engineering | Biotechnology | Biomaterials | Nucleic acids | Biomedical Engineering and Bioengineering | Chemical Bioengineering | Nucleic AcidAdditional physical formats: Printed edition:: No title; Printed edition:: No title; Printed edition:: No titleDDC classification: 610.28 Online resources: Click here to access online
Contents:
Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work.
In: Springer Nature eBookSummary: This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.
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Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work.

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

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