Introduction -- Robotic micromanipulation of small organisms -- Robotic positioning of zebrafish larva -- Robotic orientation of zebrafish larva -- Robotic immobilization of zebrafish larva -- Robotic microinjection of zebrafish larva -- Visual detection of zebrafish larva -- Trajectory tracking of zebrafish larvae group.

This book offers readers a series of robotic methods for manipulating zebrafish larva, one of the most popular model vertebrates widely used in biomedical research and clinical applications. The authors leverage advanced control theories, image processing algorithms, and artificial intelligence to establish a robot-assisted automated or semi-automated zebrafish larva-targeted micromanipulation system for different experimental purposes. The methods presented are generic and can be translated to manipulate other types of biological objects, such as embryos or cells. Coverage includes topics that span the procedures of manipulating zebrafish larva, such as in-plane positioning, three-dimensional orientation, deformation-controllable immobilization, organ-targeted microinjection, whole-organism imaging, and high-throughput trajectory tracking of zebrafish larvae group movement. Robotic Micromanipulation of Zebrafish Larva is written in a simple, clear, and easy-to-read style. It is an ideal reference for academic researchers and biomedical operators. It is also a valuable resource for students learning robotics, control and system theories, image processing, artificial intelligence, and biomedical engineering. Introduces general robotic micromanipulation methods of handling small organisms; Includes use cases of zebrafish larva; Summarizes emerging issues on robotic micromanipulation for research and applications to inspire future investigation.