000 04143nam a22005535i 4500
001 978-3-662-49118-8
003 DE-He213
005 20200420220215.0
007 cr nn 008mamaa
008 160121s2016 gw | s |||| 0|eng d
020 _a9783662491188
_9978-3-662-49118-8
024 7 _a10.1007/978-3-662-49118-8
_2doi
050 4 _aR856-857
072 7 _aMQW
_2bicssc
072 7 _aTEC009000
_2bisacsh
082 0 4 _a610.28
_223
245 1 0 _aEssentials of Single-Cell Analysis
_h[electronic resource] :
_bConcepts, Applications and Future Prospects /
_cedited by Fan-Gang Tseng, Tuhin Subhra Santra.
250 _a1st ed. 2016.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg :
_bImprint: Springer,
_c2016.
300 _aX, 414 p. 142 illus., 127 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSeries in BioEngineering,
_x2196-8861
505 0 _aSingle Cell Behavioral Assays for Heterogeneity Studies -- Systems biology in single cells -- Electroporation for single cell analysis -- Microinjection for single cell analysis -- Optical tools for single cell manipulation and analysis -- Optoelectrokinetic manipulation for cell analysis -- Continuous micro/nano-fluidic devices for single cell analysis -- Single Cell Mechanical Properties: Label-Free Biomarkers for Cell Status Evaluation -- Cytometry of single cells for biology and biomedicine -- Single cell genomics and epigenomics -- Single cell Metabolomics -- Applications of cell based drug delivery systems: use of single cell assay -- Applications of Single Cell Sequencing in Cancer -- Single Cell Characterization of Microalgal Lipid Contents with Confocal Raman Microscopy -- Single Differentiated Neurons from Pluripotent Embryonic Stem Cells: Motor Protein Modelling and Neurodegenerative Disease.
520 _aThis book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
650 0 _aEngineering.
650 0 _aBiotechnology.
650 0 _aBioinformatics.
650 0 _aCell biology.
650 0 _aBiomedical engineering.
650 1 4 _aEngineering.
650 2 4 _aBiomedical Engineering.
650 2 4 _aMicroengineering.
650 2 4 _aBioinformatics.
650 2 4 _aBiological and Medical Physics, Biophysics.
650 2 4 _aCell Biology.
700 1 _aTseng, Fan-Gang.
_eeditor.
700 1 _aSantra, Tuhin Subhra.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783662491164
830 0 _aSeries in BioEngineering,
_x2196-8861
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-662-49118-8
912 _aZDB-2-ENG
942 _cEBK
999 _c51564
_d51564