000 03076nam a22004815i 4500
001 978-3-642-39128-6
003 DE-He213
005 20200420220223.0
007 cr nn 008mamaa
008 130724s2014 gw | s |||| 0|eng d
020 _a9783642391286
_9978-3-642-39128-6
024 7 _a10.1007/978-3-642-39128-6
_2doi
050 4 _aT174.7
072 7 _aTDPB
_2bicssc
072 7 _aTEC027000
_2bisacsh
082 0 4 _a620.5
_223
100 1 _aMalboubi, Majid.
_eauthor.
245 1 0 _aGigaseal Formation in Patch Clamping
_h[electronic resource] :
_bWith Applications of Nanotechnology /
_cby Majid Malboubi, Kyle Jiang.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg :
_bImprint: Springer,
_c2014.
300 _aXI, 94 p. 81 illus., 65 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringerBriefs in Applied Sciences and Technology,
_x2191-530X
505 0 _aChapter 1 Introduction -- Chapter 2 Development of patch clamping -- Chapter 3 Gigaseal formation -- Chapter 4 Effect of roughness on gigaseal formation -- Chapter 5 Effect of hydrophilicIty on gigaseal formation -- Chapter 6 Effect of tip size on gigaseal formation -- Chapter 7 Study of glass micropipettes from tip formation to characterization -- References.
520 _aThis book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.
650 0 _aEngineering.
650 0 _aNanotechnology.
650 0 _aBiomedical engineering.
650 1 4 _aEngineering.
650 2 4 _aNanotechnology and Microengineering.
650 2 4 _aBiomedical Engineering.
700 1 _aJiang, Kyle.
_eauthor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783642391279
830 0 _aSpringerBriefs in Applied Sciences and Technology,
_x2191-530X
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-642-39128-6
912 _aZDB-2-ENG
942 _cEBK
999 _c52004
_d52004