000 03502nam a22004935i 4500
001 978-3-319-16253-9
003 DE-He213
005 20200420221254.0
007 cr nn 008mamaa
008 150314s2015 gw | s |||| 0|eng d
020 _a9783319162539
_9978-3-319-16253-9
024 7 _a10.1007/978-3-319-16253-9
_2doi
050 4 _aR856-857
072 7 _aMQW
_2bicssc
072 7 _aTEC009000
_2bisacsh
082 0 4 _a610.28
_223
100 1 _aMunschauer, Mathias.
_eauthor.
245 1 0 _aHigh-Resolution Profiling of Protein-RNA Interactions
_h[electronic resource] /
_cby Mathias Munschauer.
264 1 _aCham :
_bSpringer International Publishing :
_bImprint: Springer,
_c2015.
300 _aXXIII, 121 p. 25 illus., 20 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
505 0 _aIntroduction -- Mapping Regulatory Interactions of the Rna-Binding Protein Lin28b -- Exploring The Sequence Space Contacted by the Ensemble of Rna-Binding Proteins -- Revealing Cell-Type Specific Differences in Protein Occupancy on Rna -- Discussion.
520 _aThe work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel 'protein occupancy profiling' technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3' UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universit�at Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.  .
650 0 _aEngineering.
650 0 _aBioinformatics.
650 0 _aSystems biology.
650 0 _aBiomedical engineering.
650 1 4 _aEngineering.
650 2 4 _aBiomedical Engineering.
650 2 4 _aSystems Biology.
650 2 4 _aComputational Biology/Bioinformatics.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783319162522
830 0 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-319-16253-9
912 _aZDB-2-ENG
942 _cEBK
999 _c52770
_d52770