000			05916cam a2200553Mu 4500
001			9781315374758
003			FlBoTFG
005			20220711212845.0
006			m d
007			cr cnu---unuuu
008			200104s2019 flu o 000 0 eng d
040			_aOCoLC-P _beng _cOCoLC-P
020			_a9781439821701
020			_a1439821704
020			_a9781315374758 _q(electronic bk.)
020			_a1315374757 _q(electronic bk.)
020			_a9781315320229 _q(electronic bk. : Mobipocket)
020			_a1315320223 _q(electronic bk. : Mobipocket)
020			_a9781498752954 _q(electronic bk. : EPUB)
020			_a1498752950 _q(electronic bk. : EPUB)
035			_a(OCoLC)1134854724
035			_a(OCoLC-P)1134854724
050		4	_aQD96.F56
072		7	_aMED _x008000 _2bisacsh
072		7	_aSCI _x013060 _2bisacsh
072		7	_aSCI _x055000 _2bisacsh
072		7	_aPSB _2bicssc
082	0	4	_a543.56 _223
100	1		_aGryczynski, Zygmunt, _eauthor. _920245
245	1	0	_aPractical Fluorescence Spectroscopy _h[electronic resource].
260			_aBoca Raton : _bCRC Press LLC, _c2019.
300			_a1 online resource (793 p.)
500			_aDescription based upon print version of record.
505	0		_aCover -- Half Title -- Title Page -- Copyright Page -- Table of Contents -- Preface -- Authors -- Chapter 1: Theory of Light and Light Interaction with Matter -- 1-1 BASICS OF LIGHT -- 1-2 LIGHT POLARIZATION -- Polarizers -- 1-3 INTERACTION OF LIGHT AND CHROMOPHORES -- Absorption of Light -- Emission of Light -- 1-4 INTERACTIONS AMONG CHROMOPHORES -- Fluorescence Quenching -- Fluorescence (Förster) Resonance Energy Transfer (FRET) -- 1-5 SCATTERING -- Elastic Scattering -- Raman Scattering -- SUMMARY -- Chapter 2: Experimental Basics -- 2-1 TYPES OF CUVETTES -- 2-2 TYPES OF FILTERS
505	8		_a2-3 MEASURING TRANSMITTANCE/ABSORBANCE -- Special Cases of Measuring Absorption -- Measurements of Absorption of Low Volume Samples -- Measurement of Absorption of Small (Solid) Samples -- 2-4 FACTORS AFFECTING THE PRECISION OF ABSORPTION MEASUREMENT -- 2-5 EFFECT OF SAMPLE SCATTERING ON MEASURED TRANSMISSION AND ABSORBANCE -- 2-6 MEASURING EMISSION -- 2-7 EFFICIENCY OF COLLECTING EMISSION: MEASURING QUANTUM YIELD -- Quantum Yield Calculation -- How to Convert from Wavenumbers to Wavelengths? -- Absorption -- Area under the Emission Spectra -- 2-8 WAVELENGTH CALIBRATION -- 2-9 INSTRUMENT RESPONSE
505	8		_a2-10 LAMP PROFILE -- 2-11 PEAKS AND SPECTRAL SHAPES -- 2-12 STANDARD DEVIATION AND HEIGHT -- 2-13 SUMS OF PEAKS -- 2-14 NOISE-SIGNAL-TO-NOISE RATIO -- 2-15 SAMPLING RATE -- 2-16 BANDWIDTH -- 2-17 ARTIFACTS AND ERRORS -- 2-18 SPECTRAL OVERLAP -- 2-19 PEAK NORMALIZATION -- 2-20 RESIDUALS -- 2-21 INTERPOLATION -- Chapter 3: Steady State Experiments-Transmission/Absorption -- INTRODUCTION -- Introduction to Transmittance/Absorbance -- Introduction to Absorption Measurements for Chromophores in Solutions -- Measuring Absorption of Thin Samples (Films) -- EXPERIMENTS
505	8		_aExperiment 3-1 Transmittance of a Transparent Plate -- Experiment 3-2 Transmittance through Irregular (Not Square) Objects -- Experiment 3-3 Transmission of Different Cuvettes -- Experiment 3-4 Transmittance of Cuvette Filled with Air, Water, or Other Solvents-Reflections -- Experiment 3-5 Measuring Absorbance of Chromophores in Solution -- Experiment 3-6 Measuring Absorbance of Chromophores in Solutions: Absorbance Presented in Different Scales -- Experiment 3-7 Absorbance of Rhodamine and Anthracene-Normalized Absorption Spectra
505	8		_aExperiment 3-8 Absorbance of Rhodamine 800-Effect of Out of Range Absorption -- Experiment 3-9 Absorbance of Anthracene-Low Absorption Range -- Experiment 3-10 Low Absorbance-How to Improve Quality of Measured Spectrum -- Experiment 3-11 Absorbance of Rhodamine B-Proper Use of Cuvettes -- Experiment 3-12 How to Adopt a Fluorescence Cuvette for Absorbance Measurement -- Experiment 3-13 Determining the Concentration of Chromophores in a Solution from Known Optical Density (Absorption) -- Experiment 3-14 Absorbance of Benzene-Resolvable Vibrionic States
500			_aExperiment 3-15 Measuring the Transmission of Filters
520			_aPresenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements
588			_aOCLC-licensed vendor bibliographic record.
650		7	_aMEDICAL / Biochemistry _2bisacsh _910709
650		7	_aSCIENCE / Chemistry / Industrial & Technical _2bisacsh _920246
650		7	_aSCIENCE / Physics _2bisacsh _910678
650		0	_aFluorescence spectroscopy. _914614
856	4	0	_3Taylor & Francis _uhttps://www.taylorfrancis.com/books/9781315374758
856	4	2	_3OCLC metadata license agreement _uhttp://www.oclc.org/content/dam/oclc/forms/terms/vbrl-201703.pdf
942			_cEBK
999			_c72334 _d72334