000 | 04318nam a22006375i 4500 | ||
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001 | 978-981-10-6766-2 | ||
003 | DE-He213 | ||
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007 | cr nn 008mamaa | ||
008 | 171230s2018 si | s |||| 0|eng d | ||
020 |
_a9789811067662 _9978-981-10-6766-2 |
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024 | 7 |
_a10.1007/978-981-10-6766-2 _2doi |
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_a610.28 _223 |
100 | 1 |
_aHosseini, Samira. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _938064 |
|
245 | 1 | 0 |
_aEnzyme-linked Immunosorbent Assay (ELISA) _h[electronic resource] : _bFrom A to Z / _cby Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa. |
250 | _a1st ed. 2018. | ||
264 | 1 |
_aSingapore : _bSpringer Nature Singapore : _bImprint: Springer, _c2018. |
|
300 |
_aXI, 115 p. 38 illus., 34 illus. in color. _bonline resource. |
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336 |
_atext _btxt _2rdacontent |
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337 |
_acomputer _bc _2rdamedia |
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338 |
_aonline resource _bcr _2rdacarrier |
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347 |
_atext file _bPDF _2rda |
||
490 | 1 |
_aSpringerBriefs in Forensic and Medical Bioinformatics, _x2196-8853 |
|
505 | 0 | _a1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA. | |
520 | _aThis book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. . | ||
650 | 0 |
_aBiomedical engineering. _93292 |
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650 | 0 |
_aImmunology. _910726 |
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650 | 0 |
_aBiophysics. _94093 |
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650 | 0 |
_aProteins . _938065 |
|
650 | 0 |
_aBiotechnology. _97533 |
|
650 | 0 |
_aBioinformatics. _99561 |
|
650 | 1 | 4 |
_aBiomedical Engineering and Bioengineering. _931842 |
650 | 2 | 4 |
_aImmunology. _910726 |
650 | 2 | 4 |
_aBiophysics. _94093 |
650 | 2 | 4 |
_aProtein Biochemistry. _938066 |
650 | 2 | 4 |
_aBiotechnology. _97533 |
650 | 2 | 4 |
_aComputational and Systems Biology. _931619 |
700 | 1 |
_aVázquez-Villegas, Patricia. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _938067 |
|
700 | 1 |
_aRito-Palomares, Marco. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _938068 |
|
700 | 1 |
_aMartinez-Chapa, Sergio O. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _938069 |
|
710 | 2 |
_aSpringerLink (Online service) _938070 |
|
773 | 0 | _tSpringer Nature eBook | |
776 | 0 | 8 |
_iPrinted edition: _z9789811067655 |
776 | 0 | 8 |
_iPrinted edition: _z9789811067679 |
830 | 0 |
_aSpringerBriefs in Forensic and Medical Bioinformatics, _x2196-8853 _938071 |
|
856 | 4 | 0 | _uhttps://doi.org/10.1007/978-981-10-6766-2 |
912 | _aZDB-2-ENG | ||
912 | _aZDB-2-SXE | ||
942 | _cEBK | ||
999 |
_c76290 _d76290 |