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020 _a9789811067662
_9978-981-10-6766-2
024 7 _a10.1007/978-981-10-6766-2
_2doi
050 4 _aR856-857
072 7 _aMQW
_2bicssc
072 7 _aTEC059000
_2bisacsh
072 7 _aMQW
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082 0 4 _a610.28
_223
100 1 _aHosseini, Samira.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_938064
245 1 0 _aEnzyme-linked Immunosorbent Assay (ELISA)
_h[electronic resource] :
_bFrom A to Z /
_cby Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa.
250 _a1st ed. 2018.
264 1 _aSingapore :
_bSpringer Nature Singapore :
_bImprint: Springer,
_c2018.
300 _aXI, 115 p. 38 illus., 34 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringerBriefs in Forensic and Medical Bioinformatics,
_x2196-8853
505 0 _a1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.
520 _aThis book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. .
650 0 _aBiomedical engineering.
_93292
650 0 _aImmunology.
_910726
650 0 _aBiophysics.
_94093
650 0 _aProteins .
_938065
650 0 _aBiotechnology.
_97533
650 0 _aBioinformatics.
_99561
650 1 4 _aBiomedical Engineering and Bioengineering.
_931842
650 2 4 _aImmunology.
_910726
650 2 4 _aBiophysics.
_94093
650 2 4 _aProtein Biochemistry.
_938066
650 2 4 _aBiotechnology.
_97533
650 2 4 _aComputational and Systems Biology.
_931619
700 1 _aVázquez-Villegas, Patricia.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_938067
700 1 _aRito-Palomares, Marco.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_938068
700 1 _aMartinez-Chapa, Sergio O.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_938069
710 2 _aSpringerLink (Online service)
_938070
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9789811067655
776 0 8 _iPrinted edition:
_z9789811067679
830 0 _aSpringerBriefs in Forensic and Medical Bioinformatics,
_x2196-8853
_938071
856 4 0 _uhttps://doi.org/10.1007/978-981-10-6766-2
912 _aZDB-2-ENG
912 _aZDB-2-SXE
942 _cEBK
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