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| 001 | 978-981-13-8094-5 | ||
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| 008 | 190513s2019 si | s |||| 0|eng d | ||
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_a9789811380945 _9978-981-13-8094-5 |
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_a10.1007/978-981-13-8094-5 _2doi |
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_a610.28 _223 |
| 100 | 1 |
_aSong, Seo Woo. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _945863 |
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| 245 | 1 | 0 |
_aOne-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays _h[electronic resource] / _cby Seo Woo Song. |
| 250 | _a1st ed. 2019. | ||
| 264 | 1 |
_aSingapore : _bSpringer Nature Singapore : _bImprint: Springer, _c2019. |
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| 300 |
_aXXI, 52 p. 46 illus., 40 illus. in color. _bonline resource. |
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_atext _btxt _2rdacontent |
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_acomputer _bc _2rdamedia |
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_aonline resource _bcr _2rdacarrier |
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_atext file _bPDF _2rda |
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_aSpringer Theses, Recognizing Outstanding Ph.D. Research, _x2190-5061 |
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| 505 | 0 | _aAbstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the ‘Pipetting-Free’ HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography. | |
| 520 | _aThis thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients’ primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of ‘partipetting’ for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications. | ||
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_aBiomedical engineering. _93292 |
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_aBioinformatics. _99561 |
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_aSoft condensed matter. _917418 |
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_aMicrotechnology. _928219 |
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_aMicroelectromechanical systems. _96063 |
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_aIndustrial microbiology. _98413 |
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| 650 | 1 | 4 |
_aBiomedical Engineering and Bioengineering. _931842 |
| 650 | 2 | 4 |
_aComputational and Systems Biology. _931619 |
| 650 | 2 | 4 |
_aSoft and Granular Matter. _934622 |
| 650 | 2 | 4 |
_aMicrosystems and MEMS. _945864 |
| 650 | 2 | 4 |
_aIndustrial Microbiology. _98413 |
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_aSpringerLink (Online service) _945865 |
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| 773 | 0 | _tSpringer Nature eBook | |
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_iPrinted edition: _z9789811380938 |
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_iPrinted edition: _z9789811380952 |
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_iPrinted edition: _z9789811380969 |
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_aSpringer Theses, Recognizing Outstanding Ph.D. Research, _x2190-5061 _945866 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-981-13-8094-5 |
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