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020 _a9780750333511
_qebook
020 _a9780750333504
_qmobi
020 _z9780750333498
_qprint
020 _z9780750333528
_qmyPrint
024 7 _a10.1088/978-0-7503-3351-1
_2doi
035 _a(CaBNVSL)thg00083448
035 _a(OCoLC)1344491119
040 _aCaBNVSL
_beng
_erda
_cCaBNVSL
_dCaBNVSL
050 4 _aQH212.F55
_bA948 2022eb
072 7 _aPHVN
_2bicssc
072 7 _aSCI009000 $2bisacsh
082 0 4 _a570.282
_223
100 1 _aAxelrod, Daniel,
_d1948-
_eauthor.
_970868
245 1 0 _aTotal internal reflection fluorescence (TIRF) and evanescence microscopies /
_cDaniel Axelrod.
246 3 _aTIRF and evanescence microscopies.
264 1 _aBristol [England] (Temple Circus, Temple Way, Bristol BS1 6HG, UK) :
_bIOP Publishing,
_c[2022]
300 _a1 online resource (various pagings) :
_billustrations (some color).
336 _atext
_2rdacontent
337 _aelectronic
_2isbdmedia
338 _aonline resource
_2rdacarrier
490 1 _a[IOP release $release]
490 1 _aBiophysical Society-IOP series
490 1 _aIOP ebooks. [2022 collection]
500 _a"Version: 20220901"--Title page verso.
504 _aIncludes bibliographical references.
505 0 _a1. Introduction to optical evanescence -- 1.1. Overview -- 1.2. Applications to biochemistry and cell biology -- 1.3. Ray picture of total internal reflection -- 1.4. Maxwell's equations and wave numbers -- 1.5. Causes of evanescence : a physical view
505 8 _a2. Total internal reflection theory -- 2.1. Rays and TIR -- 2.2. Waves and TIR -- 2.3. Evanescent intensity -- 2.4. Finite-width incident beams : the Goos-H�anchen shift -- 2.5. Reflected intensities
505 8 _a3. Structure in the lower-index material -- 3.1. Light absorption in medium 1 -- 3.2. Intermediate layers -- 3.3. Metal films and surface plasmons -- 3.4. Slab waveguides -- 3.5. Total internal reflection scattering
505 8 _a4. Emission of fluorophores near a surface -- 4.1. The emission near field : a semi-qualitative view -- 4.2. Capture of the near field : summary of quantitative theory -- 4.3. Polarization of the emitted electric field -- 4.4. Emitted intensity and total power -- 4.5. Emitted intensity vs polar angle -- 4.6. Total fluorescence collection through a microscope objective -- 4.7. Pattern at the back focal plane -- 4.8. Characterization of films with supercritical-emission light -- 4.9. Effect of metal films on fluorescence emission -- 4.10. Pattern at the image plane -- 4.11. Virtual supercritical angle fluorescence microscopy (vSAF) -- 4.12. Emission polarization including supercritical light -- 4.13. SAF/UAF : measurement of the absolute distance between a fluorophore and a surface -- 4.14. Effect of near-field capture on fluorescence lifetime
505 8 _a5 Optical configurations and setup -- 5.1. Inverted microscope TIR with prism above -- 5.2. Inverted microscope TIR with prism below -- 5.3. Upright microscope TIR with prism below -- 5.4. Objective-based TIR -- 5.5. Incidence angle, multicolor, and polarization control -- 5.6. Alignment -- 5.7. Rapid chopping between TIR and epi-illumination -- 5.8. Supercritical-angle fluorescence (SAF) emission setup -- 5.9. Imaging the back focal plane directly -- 5.10. Measurement of evanescent field depth -- 5.11. TIRF-structured illumination microscopy (TIRF-SIM)
505 8 _a6. Applications of TIRF microscopy and its combination with other fluorescence techniques -- 6.1. Refractive indices in cell cultures -- 6.2. Axial position and motion of cell components -- 6.3. Quenching with a metal film -- 6.4. Image sharpening in TIR -- 6.5. Polarized excitation TIRF -- 6.6. Variable-depth TIRF -- 6.7. Optical force in an evanescent field -- 6.8. TIR/FCS and TIR/FRAP -- 6.9. TIR-continuous photobleaching -- 6.10. TIR-FRET -- 6.11. Two-photon TIRF.
520 3 _aThis book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applications for combinations of evanescence microscopies with other optical techniques such as polarization, photobleaching, correlation spectroscopy, scattering, image enhancement, optical force, two-photon, energy transfer, structured illumination, scanning, quenching, and single molecule detection. Physical and qualitative discussions augment the rigorous math, making the book accessible and interesting to a wide range of audiences with backgrounds from biology to chemistry to physics. The book also contains useful step-by-step guides to building, modifying, and aligning TIRF microscopy systems for specialized purposes. Part of Biophysical Society-IOP series.
521 _aResearchers who work with TIRF and near field optics as a standard technique, and those who want to develop the techniques farther.
530 _aAlso available in print.
538 _aMode of access: World Wide Web.
538 _aSystem requirements: Adobe Acrobat Reader, EPUB reader, or Kindle reader.
545 _aDaniel Axelrod is a Professor Emeritus of Physics and Biophysics at the University of Michigan, Ann Arbor. His specialty is developing novel optical microscopy techniques to study the motion and organization of biological molecules and cellular organelles near biological surfaces. Many biological aspects are done in collaboration with research groups at the University of Michigan Medical School. Professor Axelrod is a Fellow of the Biophysical Society and a recipient of the Biophysical Society's 2010 Gregorio Weber Award for fluorescence theory and applications.
588 0 _aTitle from PDF title page (viewed on October 5, 2022).
650 0 _aFluorescence microscopy.
_918965
650 0 _aTotal internal reflection (Optics)
_970869
650 7 _aBiophysics.
_2bicssc
_94093
650 7 _aMedical physics and biophysics.
_2bisacsh
_970121
710 2 _aInstitute of Physics (Great Britain),
_epublisher.
_911622
776 0 8 _iPrint version:
_z9780750333498
_z9780750333528
830 0 _aIOP (Series).
_pRelease 22.
_970870
830 0 _aBiophysical Society-IOP series.
_970276
830 0 _aIOP ebooks.
_p2022 collection.
_970871
856 4 0 _uhttps://iopscience.iop.org/book/mono/978-0-7503-3351-1
942 _cEBK
999 _c82920
_d82920