000 | 04074nam a22005415i 4500 | ||
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001 | 978-3-031-01666-0 | ||
003 | DE-He213 | ||
005 | 20240730163648.0 | ||
007 | cr nn 008mamaa | ||
008 | 220601s2020 sz | s |||| 0|eng d | ||
020 |
_a9783031016660 _9978-3-031-01666-0 |
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024 | 7 |
_a10.1007/978-3-031-01666-0 _2doi |
|
050 | 4 | _aT1-995 | |
072 | 7 |
_aTBC _2bicssc |
|
072 | 7 |
_aTEC000000 _2bisacsh |
|
072 | 7 |
_aTBC _2thema |
|
082 | 0 | 4 |
_a620 _223 |
100 | 1 |
_aBuonincontri, Guido. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _979795 |
|
245 | 1 | 0 |
_a3D Electro-Rotation of Single Cells _h[electronic resource] / _cby Guido Buonincontri, Liang Huang, Wenhui Wang. |
250 | _a1st ed. 2020. | ||
264 | 1 |
_aCham : _bSpringer International Publishing : _bImprint: Springer, _c2020. |
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300 |
_aXVII, 101 p. _bonline resource. |
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336 |
_atext _btxt _2rdacontent |
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337 |
_acomputer _bc _2rdamedia |
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338 |
_aonline resource _bcr _2rdacarrier |
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347 |
_atext file _bPDF _2rda |
||
490 | 1 |
_aSynthesis Lectures on Biomedical Engineering, _x1930-0336 |
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505 | 0 | _aAcknowledgments -- Introduction -- Thick-Electrode DEP for Single-Cell 3D Rotation -- Opto-Electronic Integration of Thick-Electrode DEP Microfluidic Chip -- Summary and Outlook -- References -- Authors' Biographies. | |
520 | _aDielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis. | ||
650 | 0 |
_aEngineering. _99405 |
|
650 | 0 |
_aBiophysics. _94093 |
|
650 | 0 |
_aBiomedical engineering. _93292 |
|
650 | 1 | 4 |
_aTechnology and Engineering. _979796 |
650 | 2 | 4 |
_aBiophysics. _94093 |
650 | 2 | 4 |
_aBiomedical Engineering and Bioengineering. _931842 |
700 | 1 |
_aHuang, Liang. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _979797 |
|
700 | 1 |
_aWang, Wenhui. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _979798 |
|
710 | 2 |
_aSpringerLink (Online service) _979799 |
|
773 | 0 | _tSpringer Nature eBook | |
776 | 0 | 8 |
_iPrinted edition: _z9783031000454 |
776 | 0 | 8 |
_iPrinted edition: _z9783031005381 |
776 | 0 | 8 |
_iPrinted edition: _z9783031027949 |
830 | 0 |
_aSynthesis Lectures on Biomedical Engineering, _x1930-0336 _979800 |
|
856 | 4 | 0 | _uhttps://doi.org/10.1007/978-3-031-01666-0 |
912 | _aZDB-2-SXSC | ||
942 | _cEBK | ||
999 |
_c84851 _d84851 |