000 04074nam a22005415i 4500
001 978-3-031-01666-0
003 DE-He213
005 20240730163648.0
007 cr nn 008mamaa
008 220601s2020 sz | s |||| 0|eng d
020 _a9783031016660
_9978-3-031-01666-0
024 7 _a10.1007/978-3-031-01666-0
_2doi
050 4 _aT1-995
072 7 _aTBC
_2bicssc
072 7 _aTEC000000
_2bisacsh
072 7 _aTBC
_2thema
082 0 4 _a620
_223
100 1 _aBuonincontri, Guido.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_979795
245 1 0 _a3D Electro-Rotation of Single Cells
_h[electronic resource] /
_cby Guido Buonincontri, Liang Huang, Wenhui Wang.
250 _a1st ed. 2020.
264 1 _aCham :
_bSpringer International Publishing :
_bImprint: Springer,
_c2020.
300 _aXVII, 101 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSynthesis Lectures on Biomedical Engineering,
_x1930-0336
505 0 _aAcknowledgments -- Introduction -- Thick-Electrode DEP for Single-Cell 3D Rotation -- Opto-Electronic Integration of Thick-Electrode DEP Microfluidic Chip -- Summary and Outlook -- References -- Authors' Biographies.
520 _aDielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.
650 0 _aEngineering.
_99405
650 0 _aBiophysics.
_94093
650 0 _aBiomedical engineering.
_93292
650 1 4 _aTechnology and Engineering.
_979796
650 2 4 _aBiophysics.
_94093
650 2 4 _aBiomedical Engineering and Bioengineering.
_931842
700 1 _aHuang, Liang.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_979797
700 1 _aWang, Wenhui.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_979798
710 2 _aSpringerLink (Online service)
_979799
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9783031000454
776 0 8 _iPrinted edition:
_z9783031005381
776 0 8 _iPrinted edition:
_z9783031027949
830 0 _aSynthesis Lectures on Biomedical Engineering,
_x1930-0336
_979800
856 4 0 _uhttps://doi.org/10.1007/978-3-031-01666-0
912 _aZDB-2-SXSC
942 _cEBK
999 _c84851
_d84851